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image lab touch software version 6 0 1 bio rad  (Bio-Rad)


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    Bio-Rad image lab touch software version 6 0 1 bio rad
    Image Lab Touch Software Version 6 0 1 Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+chemidoc+image+lab+v+6+0+1/Image+Lab+Touch+Software+for+ChemiDoc+Imagers+Version+2%2E4/pmc12039166-67-17-23
    Average 99 stars, based on 193 article reviews
    image lab touch software version 6 0 1 bio rad - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.

    Mutagenesis:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.

    Control:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.

    Recombinant:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.

    Imaging:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.

    Western Blot:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.

    Activity Assay:

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.
    Article Snippet: Joosten from the Laboratory of Phytopathology, University of Wageningen.Joosten from the Laboratory of Phytopathology, University of Wageningen.. Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.. Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.Relative fluorescence units (RFU) were calculated by dividing the adjusted volume (background already subtracted) by the selected area.



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    Fig. 2 Heterologous expression of Ribonuclease T1 (Ribo1) orthologs in Nicotiana benthamiana. (a) By using the Agrobacterium tumefaciens-mediated transient expression assay, Ustilago hordei (Uh), Ustilago maydis (Um), Sporisorium reilianum (Sr), and Fusarium verticillioides (Fv) Ribo1 and active site mutant of UhRibo1 were transiently expressed in Nicotiana benthamiana leaves under the control of 35SCaMV promoter and with or without plant signal peptide (SP) for extracellular secretion. Expression of UhRibo1, UmRibo1, SrRibo1 and FvRibo1 with or without signal peptide results in plant cell death in N. benthamiana (necrotic regions). Pictures were taken after 5 d postinfection (dpi). Autofluorescence pictures were taken with Bio-Rad <t>ChemiDoc</t> ima- ging system. (b) Heterologous expression of active site mutant version of UhRibo1 in N. benthamiana. Wild-type and active site mutant of UhRibo1 were transiently expressed in N. benthamiana leaves under the control of 35SCaMV promoter and with or without plant signal peptide (SP). The schematic dia- gram depicts SP, active sites (black arrows) and HA-tag of full-length recombinant Ribo1 protein. While secreted wild-type (+SP), nonsecreted UhRibo1 (sp) and secreted active site mutant version of UhRibo1 (Ribo1M) induced plant cell death, nonsecreted active site mutant Ribo1M did not induce cell death. Pictures were taken after 5 dpi. Autofluorescence pictures were taken with Bio-Rad ChemiDoc imaging system. All pictures show representative plants of at least three biological replicates. (c) Western blot analysis showing expression of nonsecreted active site mutant version of UhRibo1 at 2 dpi. Western blot was performed with anti-HA antibody.
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    Image Search Results


    Fig. 2 Heterologous expression of Ribonuclease T1 (Ribo1) orthologs in Nicotiana benthamiana. (a) By using the Agrobacterium tumefaciens-mediated transient expression assay, Ustilago hordei (Uh), Ustilago maydis (Um), Sporisorium reilianum (Sr), and Fusarium verticillioides (Fv) Ribo1 and active site mutant of UhRibo1 were transiently expressed in Nicotiana benthamiana leaves under the control of 35SCaMV promoter and with or without plant signal peptide (SP) for extracellular secretion. Expression of UhRibo1, UmRibo1, SrRibo1 and FvRibo1 with or without signal peptide results in plant cell death in N. benthamiana (necrotic regions). Pictures were taken after 5 d postinfection (dpi). Autofluorescence pictures were taken with Bio-Rad ChemiDoc ima- ging system. (b) Heterologous expression of active site mutant version of UhRibo1 in N. benthamiana. Wild-type and active site mutant of UhRibo1 were transiently expressed in N. benthamiana leaves under the control of 35SCaMV promoter and with or without plant signal peptide (SP). The schematic dia- gram depicts SP, active sites (black arrows) and HA-tag of full-length recombinant Ribo1 protein. While secreted wild-type (+SP), nonsecreted UhRibo1 (sp) and secreted active site mutant version of UhRibo1 (Ribo1M) induced plant cell death, nonsecreted active site mutant Ribo1M did not induce cell death. Pictures were taken after 5 dpi. Autofluorescence pictures were taken with Bio-Rad ChemiDoc imaging system. All pictures show representative plants of at least three biological replicates. (c) Western blot analysis showing expression of nonsecreted active site mutant version of UhRibo1 at 2 dpi. Western blot was performed with anti-HA antibody.

    Journal: The New phytologist

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.

    doi: 10.1111/nph.19244

    Figure Lengend Snippet: Fig. 2 Heterologous expression of Ribonuclease T1 (Ribo1) orthologs in Nicotiana benthamiana. (a) By using the Agrobacterium tumefaciens-mediated transient expression assay, Ustilago hordei (Uh), Ustilago maydis (Um), Sporisorium reilianum (Sr), and Fusarium verticillioides (Fv) Ribo1 and active site mutant of UhRibo1 were transiently expressed in Nicotiana benthamiana leaves under the control of 35SCaMV promoter and with or without plant signal peptide (SP) for extracellular secretion. Expression of UhRibo1, UmRibo1, SrRibo1 and FvRibo1 with or without signal peptide results in plant cell death in N. benthamiana (necrotic regions). Pictures were taken after 5 d postinfection (dpi). Autofluorescence pictures were taken with Bio-Rad ChemiDoc ima- ging system. (b) Heterologous expression of active site mutant version of UhRibo1 in N. benthamiana. Wild-type and active site mutant of UhRibo1 were transiently expressed in N. benthamiana leaves under the control of 35SCaMV promoter and with or without plant signal peptide (SP). The schematic dia- gram depicts SP, active sites (black arrows) and HA-tag of full-length recombinant Ribo1 protein. While secreted wild-type (+SP), nonsecreted UhRibo1 (sp) and secreted active site mutant version of UhRibo1 (Ribo1M) induced plant cell death, nonsecreted active site mutant Ribo1M did not induce cell death. Pictures were taken after 5 dpi. Autofluorescence pictures were taken with Bio-Rad ChemiDoc imaging system. All pictures show representative plants of at least three biological replicates. (c) Western blot analysis showing expression of nonsecreted active site mutant version of UhRibo1 at 2 dpi. Western blot was performed with anti-HA antibody.

    Article Snippet: Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.

    Techniques: Expressing, Mutagenesis, Control, Recombinant, Imaging, Western Blot

    Fig. 3 (a) Amino acid alignment of UhRibo1, long and short version of FvRibo1. Blue box indicates 11 amino acids from UhRibo1 that are absent in short version of FvRibo1. (b) 3D-modeling of long and short version of FvRibo1. (c) Deletion of 11 aa in UhRibo1 results in loss of cell death-inducing activity. SP FvRibo1_L (long version), SP FvRibo1_S (short version), SP UhRibo1 and SP UhRibo1M-11 aa were transiently expressed in Nicotiana benthamiana leaves under the control of 35SCaMV promoter. Pictures were taken after 5 d postinfection. Autofluorescence images were taken with a Bio-Rad ChemiDoc imaging system. All pictures show representative plants of at least three biological replicates.

    Journal: The New phytologist

    Article Title: A conserved extracellular Ribo1 with broad-spectrum cytotoxic activity enables smut fungi to compete with host-associated bacteria.

    doi: 10.1111/nph.19244

    Figure Lengend Snippet: Fig. 3 (a) Amino acid alignment of UhRibo1, long and short version of FvRibo1. Blue box indicates 11 amino acids from UhRibo1 that are absent in short version of FvRibo1. (b) 3D-modeling of long and short version of FvRibo1. (c) Deletion of 11 aa in UhRibo1 results in loss of cell death-inducing activity. SP FvRibo1_L (long version), SP FvRibo1_S (short version), SP UhRibo1 and SP UhRibo1M-11 aa were transiently expressed in Nicotiana benthamiana leaves under the control of 35SCaMV promoter. Pictures were taken after 5 d postinfection. Autofluorescence images were taken with a Bio-Rad ChemiDoc imaging system. All pictures show representative plants of at least three biological replicates.

    Article Snippet: Quantification of cell death in N. benthamiana plants was performed by using Bio-Rad ChemiDoc IMAGE LAB v.6.0.1 (Hercules, CA, USA) to measure autofluorescence caused by necrosis.

    Techniques: Activity Assay, Control, Imaging